System

Only the most advanced, state-of-the-art technologies have been carefully selected by Macrogen to facilitate large studies, whilst taking care to provide smaller-scale investigations. Partnered with NGS-tech leaders from Illumina, Pacific Biosciences, Thermo Fisher Scientific, 10X Genomics, Bionano Genomics and many others, Macrogen primarily focuses on providing total genomic solutions tailored to client’s specific needs.

System

Illumina

	Illumina
Platform	Novaseq6000	Hiseq X ten	Hiseq4000		Hiseq2500	Nextseq500	Miseq
Recommended Application
Whole Genome Resequencing (human, plant & animal)	V	V	V		V
Whole-Genome De novo Sequencing	V	V	V		V		V
Exome Sequencing	V		V		V
Targeted Gene Sequencing (amplicon, gene panel, customized target)	V		V		V	V
totalRNA Sequencing	V		V		V
mRNA Sequencing	V		V		V
smallRNA Sequencing					V
Metagenome Shotgun Sequencing	V		V		V
16S Metagenomic Sequencing	V				V		V
ChIP Sequencing	V		V		V
MBD Sequencing	V		V		V
Methylation (WGBS) Sequencing	V	V	V		V
Specifications				Rapid Mode	HT (high-throughput) Mode
Reads Length	150bpPE 100bpPE	150bpPE	150bpPE 100bpPE	250bpPE 150bpPE 100bpPE 100bpSR 50bpSR	125bpPE 100bpPE 100bpSR 50bpSR	150bpPE	300bpPE 250bpPE 150bpPE
Run Scale	3,000M reads*	700M reads*	500M~600M reads*	250M reads*	400M~500M reads*	400M reads*	20-40M reads*
Quality Score	>75% bases higher than 30 (illumina) Avg. >85% bases higher than Q30 (Macrogen)	>75% bases higher than 30 (illumina) Avg. >85% bases higher than Q30 (Macrogen)	>75% bases higher than 30 (illumina) Avg. >85% bases higher than Q30 (Macrogen)	>80% bases higher than 30 (illumina) Avg. >90% bases higher than Q30 (Macrogen)	>80% bases higher than 30 (illumina) Avg. >90% bases higher than Q30 (Macrogen)	>70% bases higher than 30 (illumina) Avg. >80% bases higher than Q30 (Macrogen)	>70% bases higher than 30 (illumina) Avg. >80% bases higher than Q30 (Macrogen)
TAT	4-6 Weeks	2-4 Weeks	4-6 Weeks	2-4 Weeks	4-6 Weeks	4-6 Weeks	4-6 Weeks

* Based on a lane
** Per run SMRT cell
V Recommended
V Highly recommended

Pacbio | Thermo Fisher Scientific

	Pacbio		Thermo Fisher Scientific
Platform	RSII	Sequel	Ion PGM	Ion Proton
Recommended Application
Whole Genome Resequencing (human, plant & animal)	V	V
Exome Sequencing	V	V	V	V
Targeted Gene Sequencing (amplicon, gene panel, customized target)	V	V	V	V
totalRNA Sequencing	V	V
mRNA Sequencing	V	V
smallRNA Sequencing
Metagenome Shotgun Sequencing
16S Metagenomic Sequencing	V	V
ChIP Sequencing	V	V
MBD Sequencing	V	V
Methylation (WGBS) Sequencing	V	V
Specifications
Reads Length	10-20Kb SMRTbell	10-20Kb SMRTbell	316, 318 Chip 200bp 400bp	PI Chip 200bp
Run Scale	50-80K reads**	150K-250K**	316, 318 Chip 200bp 400bp	PI Chip 200bp
Run Scale	50-80K reads**	150K-250K**	2-3M reads	60-80M reads
Quality Score	>99.999% (QV50) consensus accuracy with data free of systematic errors (Pacbio)	>99.999% (QV50) consensus accuracy with data free of systematic errors (Pacbio)
TAT	4-6 Weeks	4-6 Weeks	4-6 Weeks	4-6 Weeks

* Based on a lane
** Per run SMRT cell
V Recommended
V Highly recommended

Other

	Other
Platform	10X Chromium	Bionano Saphyr
Recommended Application
Single Cell Gene Expression	V
Single Cell Immune Profiling	V
Genome Sequencing	V
Exome Sequencing	V
De Novo assembly	V
Genome mapping		V
Structural Variation		V

System

Illumina

Pacbio

Thermo Fisher Scientific

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