Animal & Plant

Macrogen makes it easy to sequence even the most complex animal and plant genomes — including polyploid species — with high accuracy and fast turnaround times. It’s an ideal solution for researchers in agricultural and evolutionary genomics.

With over 28 years of experience, Macrogen combines cutting-edge technology with deep expertise to detect everything from single nucleotide polymorphisms to complex genetic variations, genetic markers, and functionally relevant genes.

Animal & Plant Whole Genome Sequencing (WGS)

	Animal & Plant Whole Genome Sequencing (WGS)
Platform	Illumina	Pacbio	Oxford Nanopore
	Novaseq X 150bpPE Novaseq6000 150bpPE	Revio	PromethION
Sample Type	gDNA, Blood**, tissue, FFPE**
Library kit type	Truseq PCR free DNA kit (350bp or 550bp insert)	HiFi Library	ONT-LSK-114
Library kit type	Truseq Nano DNA kit (350bp or 550bp insert)	10Kb SMRTbell Templates 20kb SMRTbell Templates
Sample QC Requirement	Total Amount: 100ng-2ug DIN: >7.0 Volume: >20ul	Total Amount: 5.1ugAverage size: >30K A260/280 ratio: 1>1.8 A230/280: >2.0 Volume: >20ul	Total Amount: 6.0ugAverage size: >30K

Turnaround time**	2-4 weeks*** or 4-6 weeks
Data Analysis	De novo
	• Standard data analysis – Consensus sequence assembled into contigs
	• Advanced data analysis – Gene prediction – Gene annotation

Data Analysis	Resequencing
Data Analysis	• Standard data analysis – Mapping to the current plant reference genome – Analysis of mapping statistics – SNPs and InDels calling – Variant list in Text/Excel formats • Advanced data analysis – Variant annotation : SNPs and InDels are mapped to the current versions of SNV and related Genome releases – Functional annotations : Gene, gene structure, change of amino acid and crooss-mapping to public datavases – Avariety of options for mapping algorithms, variant detection algorithms,annotations, mapping to public database, group analysis, and case-control analysis are available

Recommended Coverage/ Reads length	15X, 30X	5-10X	5-10X

* library construction platform
** TAT from sample QC passed
***TAT 2-4 Express service
**** gDNA extraction fee applies

Service Overview

Request a Quote

Request an email

Request a quote

Mouse Whole Exome Sequencing (mWES)

	Mouse Whole Exome Sequencing (mWES)
Platform	Illumina
	Novaseq X 150bpPE Novaseq6000 150bpPE
Sample Type	gDNA, Blood**, tissue, FFPE**
Librarykit type	Agilent Mouse exome enrichment kit
Sample QC Requirement	Total Amount: 0.5ug DIN: >7.0 Volume: >20ul

Turnaround time**	4-6 weeks
Data Analysis	Exome • Standard data analysis – Variant Calling(SNPs/InDels) – Variant Annotation • Advanced data analysis – Various Variant Calling Pipeline – Tumor-Normal Paired Analysis – Disease Associated Genetic Analysis
Data Analysis	Targeted (Customised capture) • Standard data analysis – Variant Calling (SNPs/InDels) – Variant Annotation • Advanced data analysis – Various Variant Calling Pipeline – Chip Concordance Analysis

Recommended Coverage/ Read numbers	100X

* library construction platform
** TAT from sample QC passed
***TAT 2-4 Express service
**** gDNA extraction fee applies

Service Overview

Request a Quote

Request an email

Sample a quote

Animal & Plant Transcriptome Sequencing

	Animal & Plant Transcriptome Sequencing
	Total RNA Sequencing	mRNA Sequencing	Small RNA Sequencing
Platform	Novaseq X 150bpPE Novaseq6000 150bpPE	Novaseq X 150bpPE Novaseq6000 150bpPE	Novaseq X
Sample Type	Total RNA, Blood**, tissue**
Sample Kit Type	Truseq stranded Total RNA with Ribo-Zero kit	Truseq stranded mRNA kit	NEBNext Small RNA library
Sample Kit Type	SMARTer Ultra Low Input total RNA Library (low input protocol)*****	SMARTer Ultra Low Input total RNA Library (low input protocol)*****
Turnaround time**	4-6 weeks
Data Analysis	Gene expression data – Quantitative expression profiles – Gene ontology analysis
	De novo sequencing • Standard data analysis – De novo Assembly Statistics – Gene expression profile – Differentially Expressed Genes(DEGs) • Advanced data analysis – Gene Annotations / Gene Set Analysis – Comparative analysis – Gene ontology analysis		•Standard data analysis – Expression Profiles – Novel smRNA – Differentially Expressed miRNA •Advanced data analysis – Advanced data analysis – Gene Set Analysis – Comparative Analysis”
	Re sequencing • Standard data analysis – Mapping Statistics – Gene expression profile – SNPs and InDels calling by mapping to reference genome • Advanced data analysis – Fusion gene, Customised analysis

Recommended Coverage/ Read numbers	30M, 60M		1-20M reads