Animal & Plant

Animals and plants, especially with high complex genomes and/or polyploidy specimens can easily be accommodated by Macrogen which offers a high level of accuracy in a very short turnaround time. Ideal for the researchers in agricultural and evolutionary genomics.

Macrogen has over 20 years of experience in the industry using the most advanced technology and know-how to provide the discovery of genetic changes from a single nucleotide polymorphism to highly complex genetic variations, genetic markers, and the identification of functional genes.

Animal & Plant Whole Genome Sequencing (WGS)

	Animal & Plant Whole Genome Sequencing (WGS)
Platform	Illumina	Illumina	Illumina
	Novaseq6000 150bpPE Hiseq Xten 150bpPE (Express service)***	RSII	Sequel
Sample Type	gDNA, Blood**, tissue, FFPE**
Library kit type	Truseq PCR free DNA kit (350bp or 550bp insert)	10Kb SMRTbell Templates	SMRTbell Express Templates
Library kit type	Truseq Nano DNA kit (350bp or 550bp insert)	20kb SMRTbell Templates
Sample QC Requirement	Concentration: >10-20ng/ul Volumne: >10-100ul Total Amount: 100ng-2ug Purity (A260/280 ratio): >1.7	Concentration: >50ng/ul Volumne: >120-160ul Total Amount: 6-8ug	Concentration: >50ng/ul Volumne: >160ul Total Amount: 8ug
	For detailed sample requirement, please refer to following link: Download full list of ‘Sample QC Criteria’
Turnaround time**	2-4 weeks*** or 4-6 weeks
Data Analysis	De novo
	• Standard data analysis – Consensus sequence assembled into contigs
	• Advanced data analysis – Gene prediction – Gene annotation

Data Analysis	Resequencing
Data Analysis	• Standard data analysis – Mapping to the current plant reference genome – Analysis of mapping statistics – SNPs and InDels calling – Variant list in Text/Excel formats • Advanced data analysis – Variant annotation : SNPs and InDels are mapped to the current versions of SNV and related Genome releases – Functional annotations : Gene, gene structure, change of amino acid and crooss-mapping to public datavases – Avariety of options for mapping algorithms, variant detection algorithms,annotations, mapping to public database, group analysis, and case-control analysis are available

Recommended Coverage/ Reads length	15X, 30X	5-10X	5-10X

* library construction platform
** TAT from sample QC passed
***TAT 2-4 Express service
**** gDNA extraction fee applies

Service Overview

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Sample requirement

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Mouse Whole Exome Sequencing (mWES)

	Mouse Whole Exome Sequencing (mWES)
Platform	Illumina
	Novaseq6000 150bpPE or 100bpPE Hiseq4000 100bpPE Hiseq2500 100bpPE
Sample Type	gDNA, Blood**, tissue, FFPE**
Librarykit type	Agilent Mouse exome enrichment kit
Sample QC Requirement	Concentration: >10-50ng/ul Volumne: >10-20ul Total Amount: 1ug Purity (A260/280 ratio): >1.7
	For detailed sample requirement, please refer to following link: Download full list of ‘Sample QC Criteria’
Turnaround time**	4-6 weeks
Data Analysis	Exome • Standard data analysis – Variant Calling(SNPs/InDels) – Variant Annotation • Advanced data analysis – Various Variant Calling Pipeline – Tumor-Normal Paired Analysis – Disease Associated Genetic Analysis
Data Analysis	Targeted (Customised capture) • Standard data analysis – Variant Calling (SNPs/InDels) – Variant Annotation • Advanced data analysis – Various Variant Calling Pipeline – Chip Concordance Analysis

Recommended Coverage/ Read numbers	100X