Animal & Plant


Animals and plants, especially with high complex genomes and/or polyploidy specimens can easily be accommodated by Macrogen which offers a high level of accuracy in a very short turnaround time. Ideal for the researchers in agricultural and evolutionary genomics.

Macrogen has over 20 years of experience in the industry using the most advanced technology and know-how to provide the discovery of genetic changes from a single nucleotide polymorphism to highly complex genetic variations, genetic markers, and the identification of functional genes.

Animal & Plant Whole Genome Sequencing (WGS)

Platform Illumina Illumina Illumina
Novaseq6000 150bpPE
Hiseq Xten 150bpPE
(Express service)***
RSII Sequel
Sample Type gDNA, Blood****, tissue****, FFPE****
Library kit type Truseq PCR free DNA kit
(350bp or 550bp insert)
10Kb SMRTbell Templates SMRTbell Express Templates
Truseq Nano DNA kit
(350bp or 550bp insert)
20kb SMRTbell Templates
Sample QC Requirement Concentration: >10-20ng/ul
Volumne: >10-100ul
Total Amount: 100ng-2ug
Purity (A260/280 ratio): >1.7
Concentration: >50ng/ul
Volumne: >120-160ul
Total Amount: 6-8ug
Concentration: >50ng/ul
Volumne: >160ul
Total Amount: 8ug
For detailed sample requirement, please refer to following link:
Download full list of ‘Sample QC Criteria’
Turnaround time** 2-4 weeks*** or 4-6 weeks
Data Analysis De novo
• Standard data analysis
– Consensus sequence assembled into contigs
• Advanced data analysis
– Gene prediction
– Gene annotation
 
Data Analysis Resequencing
• Standard data analysis
– Mapping to the current plant reference genome
– Analysis of mapping statistics
– SNPs and InDels calling
– Variant list in Text/Excel formats

• Advanced data analysis
– Variant annotation : SNPs and InDels are mapped to the current versions of SNV and related Genome releases
– Functional annotations : Gene, gene structure, change of amino acid and crooss-mapping to public datavases
– Avariety of options for mapping algorithms, variant detection algorithms,annotations, mapping to public database, group analysis, and case-control analysis are available


Recommended Coverage/
Reads length
15X, 30X 5-10X 5-10X

* library construction platform
** TAT from sample QC passed
***TAT 2-4 Express service
**** gDNA extraction fee applies

Service Overview


© 2017 - 2019 Macrogen Oceania