Whole Exome Sequencing (WES)
Exome sequencing would be the most cost-effective approach for sequencing the targeted coding region of a genome, a region that comprises 1–2% of the genome but represents 80–90% of variants found in the entire genome. Therefore, it would be a perfect solution for cancer and genetic-related studies as well as human population studies.
In comparison with WGS (whole genome sequencing), 10–20 times less output is required to analyse SNP (single nucleotide polymorphisms) and Indels, meaning the variant analysis is easier, faster and uses fewer computation resources than the WGS approach.
Commonly used human WES capture kits include Agilent Sureselect Exome Capture kit and Truseq DNA Exome kit on Hiseq and Novaseq6000 series.