Microorganism


Whether you’re working in microbial ecology, virology, microbiology, environmental science, or biomedical research — Macrogen is here to support your discovery.

We offer end-to-end solutions for microbial profiling, gene marker analysis, and full genome sequencing (both de novo and re-sequencing), with a typical turnaround time of 4 to 6 weeks.

For fungal and complex microbial genomes, our hybrid approach combines the deep coverage of Illumina short reads with the long-read power of PacBio — delivering both accuracy and completeness. For routine microbial projects, high-throughput Illumina sequencing offers a fast and cost-effective option.

Metagenome Shotgun Sequencing

Amplicon Sequencing

(16s / 18s / ITS)

Platform Illumina Pacbio Illumina Pacbio
Novaseq X 150bpPE Revio Miseq i100 plus 300bpPE
Sample Type gDNA, Blood****, tissue****, FFPE****
Library kit type Truseq PCR free DNA (Meta) kit

Truseq Nano DNa (Meta) kit

HiFi library kit

Truseq 16S amplicon protocol (Targeting V3-v4) same protocol will be use for 18S using different primer sets

Kinnex 16S Full library

Sample QC Requirement Total Amount: 100ng-1ug

DIN >3.0

Volume: >20ul

Total Amount: 10ng

Concentration: >1ng/ul

Volume: >10ul

Turnaround time** 4-6 weeks
Data Analysis Whole Genome Shotgun Sequencing

• De novo Assembly
• BlastX
• Gene Prediction
• Functional Assignment
• OTU Counting
• Statistical Analysis

16R (or 18S) rRNA Sequencing

• Global Alignment
• Taxonomic Assignment
• ASV Counting
• Comparative Analysis

Recommended Coverage/
Reads length
10M reads 50-100K reads

* library construction platform
** TAT from sample QC passed
***TAT 2-4 Express service
**** gDNA extraction fee applies

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