Custom DNA Oligos
Macrogen provides the best oligo synthesis service with the latest equipment and an automated system.
High-quality oligos are produced via a purification process by removing impurities and oligos that are non-specifically bound. The quality of all oligos is controlled by MALDI-TOF mass spectrometry.
Features
- Scales from 0.025 µmole to 1 µmole
- Quick delivery by express courier (using FedEx / DHL International shipping)
- Accurate concentration provided by the liquid handling system
- Orders, progress of synthesis, delivery, and results of synthesis can be confirmed by Laboratory Information Management System (LIMS)
- Duplex oligos (double-stranded oligos) are possible
- Free shipping: ≥ 300 bases of primers
Guaranteed Yield
Standard oligos can be synthesised for 3 ~ 130 mer
For modified oligos, up to 60 mer is recommended (Inquire for oligos of 60 mer or more)
| Guaranteed Yield (OD) | Synthesis Scale | |||
| 0.025 | 0.05 | 0.2 | 1 | |
| Desalt | 3 | – | – | – |
| MOPC | – | 6 | 8 | 20 |
| PAGE | – | 2 | 4 | 8 |
| HPLC | – | 2 | 4 | 8 |
Oligo Synthesis Purification
| Salt Free (Desalt) | MOPC (Macrogen Oligonucleotide
Purification Cartridge) |
HLPC | PAGE |
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100% QC
Release and delivery of synthesised oligos occur after OD measurement and MALDI-TOF MS analysis to ensure quality. The resulting report is shipped with the ordered oligos.
The MALDI-TOF molecular weight analysis method, which is more accurate than PAGE gel QC, is used as the quality-check method for oligos and precisely analyses oligos containing N-1 and N-2.
Oligo Modification
Various types of modification oligo synthesis services are provided using high-quality raw materials and optimized processes. HPLC and PAGE purification are available with the option of various scales.
| Fluorescent dyes | FAM,HEX,TET,JOE, TAMRA, CY3, CY5, CY3.5, CY5.5 |
| CAL Fluor560 | |
| ATTO565 NHS-ester, ROX NHS-ester, TexasRed NHS-ester | |
| Non-fluorescent modifications | Phosphorylatioin, Biotin, NH2C6, NH2C12, C3 Spacer, dSpacer |
| Thiol C6 S-S | |
| Dark quenchers | Black Hole Quencher(BHQ1, BHQ2, BHQ3) |
| Blackberry Quencher(BBQ650) | |
| Dabcyl and Eclipes quencher | |
| Internal modification | DeoxyInosine (dI) |
Dual-labelled DNA Probes
| 5′ Fluorophore | 3′ Quencher |
| FAM | TAMRA, BHQ1, Dabcyl |
| HEX | TAMRA, BHQ1, BHQ2, Dabcyl |
| TET | TAMRA, BHQ1, BHQ2, Dabcyl |
| JOE | TAMRA, BHQ1, BHQ2, Dabcyl |
| CY3 | BHQ1, BHQ2, BHQ3 |
| CY3.5 | BHQ2 |
| CY5 | BHQ2, BHQ3 |
| CY5.5 | BHQ2, BHQ3, BBQ650 |
| TAMRA | BHQ1 |
| ATTO565 NHS-ester | BHQ1, BHQ2, BBQ650 |
| ROX NHS-ester | TAMRA, BHQ2, BBQ650 |
| TexasRed NHS-ester | BHQ1, BHQ2, BBQ650 |
Premade Oligos
Premade oligo is prepared to be provided immediately when customers order oligos suitable for their various research purposes.
Features
- 5nmol of ≥ 95% pure primer (PAGE purification).
- MALDI-TOF QC
- Confirms purification by HPLC
- 70 types of primers for sequencing, 17 types of primers for microbe identification and 5 types of random primers
Premade Oligos List
Primer for Sequencing
| Primer Name | Sequence | Base |
| a-Factor | TACTATTGCCAGCATTGCTGC | 21 |
| AD Reverse | AGATGGTGCACGATGCACAG | 20 |
| AOX1 Forward | GACTGGTTCCAATTGACAAGC | 21 |
| AOX1 Reverse | GCAAATGGCATTCTGACATCC | 21 |
| BGH-R | TAGAAGGCACAGTCGAGG | 18 |
| Bluescript SK | CGCTCTAGAACTAGTGGATC | 20 |
| Bluescript KS | TCGAGGTCGACGGTATC | 17 |
| CMV-F | CGCAAATGGGCGGTAGGCGTG | 21 |
| CYC1 Reverse | GCGTGAATGTAAGCGTGAC | 19 |
| DsRed1-C | AGCTGGACATCACCTCCCACAACG | 24 |
| DsRed1-N | GTACTGGAACTGGGGGGACAG | 21 |
| EBV-RP | GTGGTTTGTCCAAACTCATC | 20 |
| EGFP-N | CGTCGCCGTCCAGCTCGACCAG | 22 |
| EGFP-C | CATGGTCCTGCTGGAGTTCGTA | 22 |
| EGFP-CF | AGCACCCAGTCCGCCCTGAGC | 21 |
| EGFP-CR | CGTCCATGCCGAGAGTG | 17 |
| EGFP-NR | CGTCGCCGTCCAGCTC | 16 |
| GAL1-Forward | AATATACCTCTATACTTTAACGTC | 24 |
| Gal4AD | TACCACTACAATAGGATG | 17 |
| GAL1 Forward | TGTATCTTATGGTACTAACTG | 23 |
| GaL4AD | TACCACTACAATGGATG | 17 |
| GLprimer1 | TGTATCTTATGGTACTGTAACTG | 23 |
| GLprimer2 | CTTTATGTTTTTGGCGTCTTCCA | 23 |
| LCO1490 | GGTCAACAATCATAAAGATATTGG | 25 |
| HCO2198 | TAACTTCAGGGTGACCAAAAAATCA | 26 |
| KAN2-FP | ACCTACAACAAAGCTCTCATCAACC | 25 |
| KAN2-RP | GCAATGTAACATCAGAGATTTTGAG | 25 |
| M13F-pUC(-40) | GTTTTCCCAGTCACGAC | 17 |
| M13R-pUC(-40) | CAGGAAACAGCTATGAC | 17 |
| M13F | GTAAAACGACGGCCAGT | 17 |
| M13R | GCGGATAACAATTTCACACAGG | 22 |
| M13FP | TGTAAAACGACGGCCAGT | 18 |
| MT Forward | CATCTCAGTGCAACTAAA | 18 |
| pGEX5 | GGCAAGCCACGTTTGGTG | 18 |
| pGEX3 | GGCAAGCCACGTTTGGTG | 20 |
| pMaIE | TCAGACTGTCGATGAAGC | 18 |
| Primer Name | Sequence | Base |
| pQE-F | CCCGAAAAGTGCCACCTG | 18 |
| pQE-R | GTTCTGAGGTCATTACTGG | 19 |
| pBAD-F | ATGCCATAGCATTTTTATCCA | 21 |
| pBAD-FP | ATGCCATAGCATTTTTATCC | 20 |
| pBAD-R | GATTTAATCTGTATCAGG | 18 |
| pTrcHis Forward | GAGGTATATATTAATGTATCG | 21 |
| pJET1.2F | CGACTCACTATAGGGAGAGCGGC | 23 |
| pJET1.2R | AAGAACATCGATTTTCCATGGCAG | 24 |
| pEGFP_N | CCGTCCAGCTCGACCAG | 17 |
| pEGFP-FP | TTTAGTGAACCGTCAGATC | 19 |
| pEGFP-RP | AACAGCTCCTCGCCCTTG | 18 |
| pBacPAC-RP | GTCTGTAAATCAACAACGC | 19 |
| pDONOR-FP | TAACGCTAGCATGGATCTC | 19 |
| pESP-RP | TCCAAAAGAAGTCGAGTGG | 19 |
| pET-24a | GGGTTATGCTAGTTATTGCTCAG | 23 |
| pET-RP | CTAGTTATTGCTCAGCGG | 18 |
| pREP-fwd | GCTCGATACAATAAACGCC | 19 |
| pRH Forward | CTGTCTCTATACTCCCCTATAG | 22 |
| pRH Reverse | CAAAATTCAATAGTTACTATCGC | 23 |
| RVprimer3 | CTAGCAAAATAGGCTGTCCC | 20 |
| RVprimer4 | GACGATAGTCATGCCCCGCG | 20 |
| SP6 | ATTTAGGTGACACTATAG | 18 |
| SV40-pArev | CCTCTACAAATGTGGTATGG | 20 |
| SV40-Promoter | GCCCCTAACTCCGCCCATCC | 20 |
| STag 18mer Primer | GAACGCCAGCACATGGAC | 18 |
| QE Promoter | CCGAAAAGTGCCACCTG | 17 |
| T3 | ATTAACCCTCACTAAAG | 17 |
| T7terminator | GCTAGTTATTGCTCAGCGG | 19 |
| T7promoter | TAATACGACTCACTATAGGG | 20 |
| T7 EEV | ATGTCGTAATAACCCCGCCCCG | 22 |
| T7 | AATACGACTCACTATAG | 17 |
| U-19mer Primer | GTTTTCCCAGTCACGACGT | 19 |
| 35S-A | AAGGGTCTTGCGAAGGATAG | 20 |
| 35S-B | AGTGGAAAAGGAAGGTGGCT | 20 |
| Primer Name | Sequence | Base |
| pQE-F | CCCGAAAAGTGCCACCTG | 18 |
| pQE-R | GTTCTGAGGTCATTACTGG | 19 |
| pBAD-F | ATGCCATAGCATTTTTATCCA | 21 |
| pBAD-FP | ATGCCATAGCATTTTTATCC | 20 |
| pBAD-R | GATTTAATCTGTATCAGG | 18 |
| pTrcHis Forward | GAGGTATATATTAATGTATCG | 21 |
| pJET1.2F | CGACTCACTATAGGGAGAGCGGC | 23 |
| pJET1.2R | AAGAACATCGATTTTCCATGGCAG | 24 |
| pEGFP_N | CCGTCCAGCTCGACCAG | 17 |
| pEGFP-FP | TTTAGTGAACCGTCAGATC | 19 |
| pEGFP-RP | AACAGCTCCTCGCCCTTG | 18 |
| pBacPAC-RP | GTCTGTAAATCAACAACGC | 19 |
| pDONOR-FP | TAACGCTAGCATGGATCTC | 19 |
| pESP-RP | TCCAAAAGAAGTCGAGTGG | 19 |
| pET-24a | GGGTTATGCTAGTTATTGCTCAG | 23 |
| pET-RP | CTAGTTATTGCTCAGCGG | 18 |
| pREP-fwd | GCTCGATACAATAAACGCC | 19 |
| pRH Forward | CTGTCTCTATACTCCCCTATAG | 22 |
| pRH Reverse | CAAAATTCAATAGTTACTATCGC | 23 |
| RVprimer3 | CTAGCAAAATAGGCTGTCCC | 20 |
| RVprimer4 | GACGATAGTCATGCCCCGCG | 20 |
| SP6 | ATTTAGGTGACACTATAG | 18 |
| SV40-pArev | CCTCTACAAATGTGGTATGG | 20 |
| SV40-Promoter | GCCCCTAACTCCGCCCATCC | 20 |
| STag 18mer Primer | GAACGCCAGCACATGGAC | 18 |
| QE Promoter | CCGAAAAGTGCCACCTG | 17 |
| T3 | ATTAACCCTCACTAAAG | 17 |
| T7terminator | GCTAGTTATTGCTCAGCGG | 19 |
| T7promoter | TAATACGACTCACTATAGGG | 20 |
| T7 EEV | ATGTCGTAATAACCCCGCCCCG | 22 |
| T7 | AATACGACTCACTATAG | 17 |
| U-19mer Primer | GTTTTCCCAGTCACGACGT | 19 |
| 35S-A | AAGGGTCTTGCGAAGGATAG | 20 |
| 35S-B | AGTGGAAAAGGAAGGTGGCT | 20 |
Primer for Microbe Identification
| Primer Name | Sequence | Base |
| 27F | AGAGTTTGATCMTGGCTCAG | 20 |
| 337F | GACTCCTACGGGAGGCWGCAG | 21 |
| 518F | CCAGCAGCCGCGGTAATACG | 20 |
| 785F | GGATTAGATACCCTGGTA | 18 |
| 800R | TACCAGGGTATCTAATCC | 18 |
| 907R | CCGTCAATTCMTTTRAGTTT | 20 |
| 1100R | GGGTTGCGCTCGTTG | 15 |
| 1492R | TACGGYTACCTTGTTACGACTT | 22 |
| NS1 | GTAGTCATATGCTTGTCTC | 19 |
| NS8 | TCCGCAGGTTCACCTACGGA | 20 |
| LR0R | ACCCGCTGAACTTAAGC | 17 |
| LR7 | TACTACCACCAAGATCT | 17 |
| ITS1 | TCCGTAGGTGAACCTGCGG | 19 |
| ITS2 | GCTGCGTTCTTCATCGATGC | 20 |
| ITS3 | GCATCGATGAAGAACGCAGC | 20 |
| ITS4 | TCCTCCGCTTATTGATATGC | 20 |
| ITS5 | GGAAGTAAAAGTCGTAACAAGG | 22 |
Random Primer
| Primer Name | Sequence | Base |
| Oligo dT(15) | TTTTTTTTTTTTTTT | 15 |
| Oligo dT(18) | TTTTTTTTTTTTTTTTTT | 18 |
| Oligo dT(20) | TTTTTTTTTTTTTTTTTTTT | 20 |
| Random Hexamer | NNNNNN | 6 |
| Random Nonamer | NNNNNNNNN | 9 |
Gene Synthesis Service
The gene synthesis service synthesises genes according to the customer’s gene sequence order. It is widely used in areas where recombinant DNA is studied, such as vaccine manufacturing, gene treatment and character expression.
Macrogen’s gene synthesis service provides synthesis services for genes in a plasmid DNA state with a free cloning service, as well as DNA sequencing. Customers can use a wider range of synthesis services via the mutagenesis service. Moreover, a 100% sequence is guaranteed based on ABI 3730xl sequencing equipment, and convenient ordering and inquiries for quotations are possible through the Laboratory Information Management System (LIMS).
Features
- Free cloning service (Macrogen standard vector)
- 100% sequence guaranteed using ABI 3730xl
- Mutagenesis service available
- Provided as plasmid DNA
- Free sequencing provided
- Quotation inquiries and orders possible using Laboratory Information Management System (LIMS)
